Formate metabolism in micropigs

The toxicity of methanol is directly related to the accumulation of formate which, in turn, is related to the adequacy of the folate-dependent metabolism of formate to carbon dioxide. Thus, humans who possess low hepatic folates and low 10-CHO H4folate dehydrogenase activity metabolize formate poorl...

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Bibliographic Details
Published inToxicology and applied pharmacology Vol. 116; no. 1; p. 142
Main Authors Tephly, T R, Green, M D, Gamble, J
Format Journal Article
LanguageEnglish
Published United States 01.09.1992
Subjects
Animals
Carbon Dioxide - analysis
Carbon Radioisotopes
Formates - administration & dosage
Formates - blood
Formates - metabolism
Humans
Liver - enzymology
Liver - metabolism
Male
Mice
Oxidoreductases Acting on CH-NH Group Donors - analysis
Rats
Species Specificity
Swine
Swine, Miniature
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Summary:The toxicity of methanol is directly related to the accumulation of formate which, in turn, is related to the adequacy of the folate-dependent metabolism of formate to carbon dioxide. Thus, humans who possess low hepatic folates and low 10-CHO H4folate dehydrogenase activity metabolize formate poorly and are sensitive to methanol. Conversely, most laboratory species do not exhibit methanol toxicity because they metabolize formate at high rates. Studies reported here show that the Yucatan micropig has the lowest hepatic folates of any animal species studied. Formate oxidation rates in micropigs were 23% of rates reported for rats. The half-life of formate disappearances from the blood was 74 min, a value twice that reported for rats. In addition, 10-CHO H4folate dehydrogenase activity and amount in micropig liver is markedly reduced. Micropigs may prove useful in studies of methanol poisoning due to their low capacity for formate oxidation and their reasonable size and ease in handling.
ISSN:0041-008X
DOI:10.1016/0041-008X(92)90155-L