Initiation of cytotoxic T-cell response and protection of Balb/c mice by vaccination with an experimental ISCOMs respiratory syncytial virus subunit vaccine

• Published in: Vaccine Vol. 10; no. 2; pp. 107 - 112
• Main Authors: Trudel, M., Nadon, F., Séguin, C., Brault, S., Lusignan, Y., Lemieux, S.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1992; Elsevier
• Subjects: Animals; Antibodies, Viral - biosynthesis; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; humoral response; Interleukin-2 - biosynthesis; intramuscular; intranasal; ISCOMs vaccine; Mice; Mice, Inbred BALB C; Microbiology; Respiratory syncytial virus; Respiratory Syncytial Viruses - immunology; T-cell response; T-Lymphocytes, Cytotoxic - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - immunology; Vaccines, Synthetic - isolation & purification; Viral Vaccines - immunology; Viral Vaccines - isolation & purification; Virology; intramuscular; intranasal; Respiratory syncytial virus; ISCOMs vaccine; T-cell response; humoral response; Immune response; Antibody; Vaccination; Rodentia; Cytotoxicity; Cellular immunity; Route of administration; Paramyxoviridae; Infection; Virus; Vertebrata; Mammalia; Mouse; Viral disease; T-Lymphocyte; Pneumovirus; Humoral immunity
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/0264-410X(92)90026-G

Respiratory syncytial virus is an important human pathogen causing serious lower respiratory tract infections of children and elderly people. Previous studies on the development of experimental subunit vaccines either expressed by recombinant DNA technology or prepared from purified viral proteins adsorbed on adjuvant (ISCOMs) have shown promise. The present work reports on the effectiveness of an experimental ISCOMs vaccine in initiating humoral and cell-mediated immune responses and in providing overall protection upon live virus challenge in Balb/c mice; results indicate that vaccination by the intramuscular route is more effective, even if vaccination by the intranasal route also significantly reduced virus shedding.