Epigenetic regulation of maspin expression in the human placenta
Maspin, a tumour suppressor gene, is differentially expressed in the human placenta. Decreased expression of maspin in the first trimester corresponds with the period of maximum trophoblast invasion, suggesting a role in cell invasion and motility. Although methylation of CpG islands regulates maspi...
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Published in | Molecular human reproduction Vol. 12; no. 10; pp. 611 - 617 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
01.10.2006
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | Maspin, a tumour suppressor gene, is differentially expressed in the human placenta. Decreased expression of maspin in the first trimester corresponds with the period of maximum trophoblast invasion, suggesting a role in cell invasion and motility. Although methylation of CpG islands regulates maspin expression in cancer cells, the mechanism of maspin regulation in the human placenta is unknown. Our objectives were to determine the role of epigenetic alterations in the regulation of maspin expression in the placenta. Placental samples obtained from 7 to 40 weeks’ gestation were used for bisulphite sequencing and chromatin immunoprecipitation (ChIP) PCR. There was no significant change in the methylation indices in the promoter region of maspin throughout gestation. The levels of histone modifications associated with transcriptionally active chromatin were significantly different in placental tissues from second and third trimester relative to those from first trimester. Addition of trichostatin A (TSA) to placental explants increased the maspin mRNA expression (8- to 20-fold), whereas addition of 5-aza-cytidine (5-AzaC) had no effect on maspin expression. Our data suggest that maspin expression in the human placenta is regulated by changes in histone tail modifications. This is the first report of selective histone modifications associated with differential placental gene expression in human gestation. |
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Bibliography: | ark:/67375/HXZ-F6J7JK80-F istex:1CB7BA0E2FBB2F607D523860F55DF0956443A5AC local:074 4To whom correspondence should be addressed at: Department of Neurosurgery, 200 B, EMRB, University of Iowa, Iowa City, IA 52242, USA. E-mail: anup-madan@uiowa.edu ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1360-9947 1460-2407 |
DOI: | 10.1093/molehr/gal074 |