Immunocytochemical localization of neuromedin K (neurokinin B) in rat spinal ganglia and cord

• Published in: Peptides (New York, N.Y. : 1980) Vol. 12; no. 3; pp. 431 - 443
• Main Authors: Too, H.P., Maggio, J.E.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.05.1991; Elsevier Science
• Subjects: Animals; Biological and medical sciences; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Dorsal root ganglia; Female; Fundamental and applied biological sciences. Psychology; Ganglia, Spinal - metabolism; Immunocytochemistry; Immunohistochemistry; Male; Neurokinin A - metabolism; Neurokinin B - metabolism; Neuromedin K; Rats; Rats, Inbred Strains; Spinal cord; Spinal Cord - metabolism; Substance P; Substance P - metabolism; Tachykinins; Tissue Distribution; Vertebrates: nervous system and sense organs; Spinal cord; Dorsal root ganglia; Neuromedin K; Substance P; Immunocytochemistry; Tachykinins; Rat; Rodentia; Central nervous system; Neuropeptide; Vertebrata; Mammalia; Spinal ganglion; Localization; Tachykinin
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/0196-9781(91)90081-Y

Specific antisera directed against substance P and neuromedin K (neurokinin B) have been used in double-label immunofluorescence studies to unambiguously localize these two neuropeptides of the tachykinin family in single tissue sections of rat spinal cord and dorsal root ganglia. Substance P-like immunoreactivity (SPLI) is present but neuromedin K-like immunoreactivity (NMKLI) is undetectable in dorsal root ganglia. Both peptides are present in the spinal cord, but NMKLI is largely restricted to the dorsal gray while SPLI shows a broader distribution. In the spinal gray, NMKLI coexists with SPLI in some, but not all, fibers. While substance P in the dorsal spinal cord is largely of primary afferent origin, neuromedin K appears to originate largely from intrinsic spinal neurons.