A photoetched cell relocation matrix for long-term, quantitative observations of selected individual neurons in culture

A photoetched matrix of indium tin oxide (ITO) on glass has been developed and tested as a tool to assist in the relocation and identification of individual neuronal cells in culture. The matrix is formed by 10-15 micron wide and 300 A thick ITO lines which subdivide a 1-cm2 area into 625 smaller sq...

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Bibliographic Details
Published inJournal of neuroscience methods Vol. 14; no. 3; p. 211
Main Authors Lucas, J H, Kirkpatrick, J B, Gross, G W
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1985
Subjects
Animals
Cell Aggregation
Cell Movement
Cells, Cultured
Cytological Techniques - instrumentation
Mice - embryology
Microscopy, Interference
Neurology - instrumentation
Neurology - methods
Neurons - cytology
Spinal Cord - cytology
Spinal Cord - embryology
Time Factors
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Summary:A photoetched matrix of indium tin oxide (ITO) on glass has been developed and tested as a tool to assist in the relocation and identification of individual neuronal cells in culture. The matrix is formed by 10-15 micron wide and 300 A thick ITO lines which subdivide a 1-cm2 area into 625 smaller squares. Each of the smaller squares measures 400 micron on a side and contains a photoetched two-letter "address". The address code allows precise relocation of specific regions of a culture as well as verification of the identities of individual neurons selected for repeated observation. Marks at 50 micron intervals along the sides of the address squares permit quantitative analysis of morphological changes, cell migration, reaggregation, etc. The ITO is transparent and does not interfere with visualization of even fine details of cells with high power microscopy.
ISSN:0165-0270
DOI:10.1016/0165-0270(85)90037-8