Comparison of the nucleotide sequences of diabetogenic and nondiabetogenic encephalomyocarditis virus

• Published in: Virology (New York, N.Y.) Vol. 166; no. 2; pp. 603 - 607
• Main Authors: Cohen, Stuart H., Naviaux, Robert K., Vanden Brink, Kurt M., Jordan, George W.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.10.1988; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - microbiology; Encephalomyocarditis virus - genetics; Epidemiology; Fundamental and applied biological sciences. Psychology; Mice; Microbiology; Molecular Sequence Data; Peptide Hydrolases - metabolism; Sequence Homology, Nucleic Acid; Virology; Virus; Variant; Cardiovirus; EMC virus; RNA; Nucleotide sequence; Picornaviridae; Diabetes mellitus; Comparative study
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(88)90534-X

The nucleotide sequences of a diabetogenic variant of encephalomyocarditis (EMC) virus (D variant, ifp − phenotype) and a nondiabetogenic variant of EMC virus (B variant, ifp + phenotype) have been compared. These variants differ at eight sites. The poly(C) tract of EMC-B is three bases shorter than that of EMC-D. Of the seven sites at which nucleotide substitutions were confirmed using RNA templates, four resulted in amino acid changes in three different proteins, the leader peptide, t B (VP2), and 1D (VPI ). The biological significance of these differences can now be investigated.