Relationship between surface H-2 concentration, size of different target cells, and lysis by cytotoxic T cells

We have examined the parameters of target cell cross-sectional area and surface H-2 concentration in relation to their susceptibility to cytotoxic T-cell-mediated lysis, using a series of commonly used murine target cells of the H-2 k haplotype. We used a sensitive immunoferritin labeling technique...

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Bibliographic Details
Published inCellular immunology Vol. 98; no. 2; pp. 525 - 532
Main Authors King, N.J.C., Mullbacher, A., Blanden, R.V.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 01.04.1986
Elsevier
Subjects
Analysis of the immune response. Humoral and cellular immunity
Animals
Biological and medical sciences
Cell Cycle
cell size
cell surface
Chromium Radioisotopes
Concanavalin A - pharmacology
cytotoxicity
Cytotoxicity, Immunologic
Fundamental and applied biological sciences. Psychology
Fundamental immunology
H-2 Antigens - analysis
histocompatibility antigen H-2
Immunobiology
lymphocytes T
Mice
Mice, Inbred Strains
Organs and cells involved in the immune response
Spleen - immunology
T-Lymphocytes, Cytotoxic - immunology
Immune response
Major histocompatibility system
Rodentia
Cytotoxicity
Cellular immunity
H-2-System
Antigen
Vertebrata
Mammalia
Mouse
Target cell
T»-Lymphocyte
Haplotype
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Summary:We have examined the parameters of target cell cross-sectional area and surface H-2 concentration in relation to their susceptibility to cytotoxic T-cell-mediated lysis, using a series of commonly used murine target cells of the H-2 k haplotype. We used a sensitive immunoferritin labeling technique and electron microscopy to estimate relative cell diameter and H-2 concentration combined with standard 51Cr-release assays for cytotoxicity. We found that susceptibility to cytotoxic T-cell lysis was not related consistently to either factor alone, but was related to a combination of the two, such that above a certain value for the product of the two factors, no further increase in cytotoxic T-cell efficiency was seen. The information presented here should be of value to workers seeking to select a target cell type that will maximize the sensitivity of 51Cr-release-based assays for the detection of cytotoxic T cells.
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ISSN:0008-8749
1090-2163
DOI:10.1016/0008-8749(86)90311-4