Efficient preparation of human monoclonal antibody-secreting heterohybridomas using peripheral B lymphocytes cultured in the CD40 system

• Published in: Journal of immunological methods Vol. 159; no. 1; pp. 139 - 143
• Main Authors: Darveau, André, Chevrier, Marie-Claire, Néron, Sonia, Delage, Robert, Lemieux, Réal
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26.02.1993; Elsevier
• Subjects: Animals; Antibodies, immunoglobulins; Antibodies, Monoclonal - biosynthesis; Antigens, CD - physiology; Antigens, Differentiation, B-Lymphocyte - physiology; B-Lymphocytes - immunology; Biological and medical sciences; CD40 Antigens; CD40 system; Cell Transformation, Viral; Cells, Cultured; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Herpesvirus 4, Human; human; Humans; Hybridoma; Hybridomas - immunology; Mice; Molecular immunology; Monoclonal antibodies; Monoclonal antibody; Peripheral B lymphocyte; Hybridoma; Peripheral B lymphocyte; Monoclonal antibody; human; CD40 system; Cell culture; Vertebrata; Mammalia; Cell line; Mouse; Rodentia; Production; B-Lymphocyte; Method; Myeloma
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/0022-1759(93)90151-V

The use of peripheral B lymphocytes in the successful preparation of human monoclonal antibodies by hybridoma technology is highly dependent on lymphocyte activation procedures. We studied the ability of peripheral human B lymphocytes cultured in vitro and activated through their CD40 antigen (CD40 system) (Banchereau et al., 1991) to form antibody-secreting heterohybridomas after fusion with murine X63Ag8.653 myeloma cells. The frequency of antibody-secreting heterohybridomas formation was greatly increased (15 times) by culture of B cells in the CD40 system. The CD40 system offers many advantages over other procedures of B lymphocyte activation representing a significant technological advance in the preparation of human monoclonal antibodies by standard hybridoma technology.