Presence of a trs-Like Motif Promotes Rep-Mediated Wild-Type Adeno-Associated Virus Type 2 Integration

High-throughput integration site (IS) analysis of wild-type adeno-associated virus type 2 (wtAAV2) in human dermal fibroblasts (HDFs) and HeLa cells revealed that juxtaposition of a Rep binding site (RBS) and terminal resolution site (trs)-like motif leads to a 4-fold-increased probability of wtAAV...

Full description

Saved in:
Bibliographic Details
Published inJournal of virology Vol. 89; no. 14; pp. 7428 - 7432
Main Authors Petri, Karl, Gabriel, Richard, Agundez, Leticia, Fronza, Raffaele, Afzal, Saira, Kaeppel, Christine, Linden, R Michael, Henckaerts, Els, Schmidt, Manfred
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.07.2015
Subjects
Adeno-associated virus 2
Amino Acid Motifs
Cell Line
Dependovirus - physiology
DNA-Binding Proteins - metabolism
Epithelial Cells - virology
Fibroblasts - virology
Humans
Viral Proteins - metabolism
Virus Integration
Virus-Cell Interactions
Online AccessGet full text

Cover

More Information
Summary:High-throughput integration site (IS) analysis of wild-type adeno-associated virus type 2 (wtAAV2) in human dermal fibroblasts (HDFs) and HeLa cells revealed that juxtaposition of a Rep binding site (RBS) and terminal resolution site (trs)-like motif leads to a 4-fold-increased probability of wtAAV integration. Electrophoretic mobility shift assays (EMSAs) confirmed binding of Rep to off-target RBSs. For the first time, we show Rep protein off-target nicking activity, highlighting the importance of the nicking substrate for Rep-mediated integration.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
K.P., R.G., and L.A. contributed equally to this article.
Citation Petri K, Gabriel R, Agundez L, Fronza R, Afzal S, Kaeppel C, Linden RM, Henckaerts E, Schmidt M. 2015. Presence of a trs-like motif promotes Rep-mediated wild-type adeno-associated virus type 2 integration. J Virol 89:7428–7432. doi:10.1128/JVI.00426-15.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.00426-15