Supravital staining of eosinophils

• Published in: International journal for parasitology Vol. 26; no. 4; pp. 445 - 446
• Main Authors: Stankiewicz, M., Jonas, W., Hadas, E., Cabaj, W., Douch, P.G.C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.1996; Elsevier Science
• Subjects: Acridine Orange; Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; Cell Nucleus - ultrastructure; Cytoplasmic Granules - physiology; Cytoplasmic Granules - ultrastructure; Eosinophils; Eosinophils - chemistry; Eosinophils - cytology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; immunity; Immunobiology; Intestinal Mucosa - cytology; Intestine, Small; Leukocyte Count; leukocytes; Organs and cells involved in the immune response; Sheep; Staining and Labeling - methods; supravital staining; leukocytes; immunity; supravital staining; sheep; Eosinophils; Cell motility; Vertebrata; Mammalia; Degranulation; Sheep; Artiodactyla; Vital staining; Immunity; In vitro; Ungulata; Eosinophil

Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using th...