Supravital staining of eosinophils

Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using th...

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Bibliographic Details
Published inInternational journal for parasitology Vol. 26; no. 4; pp. 445 - 446
Main Authors Stankiewicz, M., Jonas, W., Hadas, E., Cabaj, W., Douch, P.G.C.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.04.1996
Elsevier Science
Subjects
Acridine Orange
Analysis of the immune response. Humoral and cellular immunity
Animals
Biological and medical sciences
Cell Nucleus - ultrastructure
Cytoplasmic Granules - physiology
Cytoplasmic Granules - ultrastructure
Eosinophils
Eosinophils - chemistry
Eosinophils - cytology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
immunity
Immunobiology
Intestinal Mucosa - cytology
Intestine, Small
Leukocyte Count
leukocytes
Organs and cells involved in the immune response
Sheep
Staining and Labeling - methods
supravital staining
leukocytes
immunity
supravital staining
sheep
Eosinophils
Cell motility
Vertebrata
Mammalia
Degranulation
Sheep
Artiodactyla
Vital staining
Immunity
In vitro
Ungulata
Eosinophil
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Summary:Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0020-7519
1879-0135
DOI:10.1016/0020-7519(96)00003-3