Assay of paracetamol and its metabolites in urine, plasma and saliva of children with chronic liver disease

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 13; no. 8; pp. 1033 - 1039
• Main Authors: Al-Obaidy, S.S., Li Wan Po, A., McKiernan, P.J., Glasgow, J.F.T., Millership, J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.1995; Elsevier Science
• Subjects: Acetaminophen - analysis; Acetaminophen - pharmacokinetics; Adult; Analgesics; Assay; Biological and medical sciences; Child; Child, Preschool; Chromatography, High Pressure Liquid; Chronic Disease; Humans; Infant; Liver disease; Liver Diseases - blood; Liver Diseases - metabolism; Liver Diseases - urine; Medical sciences; Neuropharmacology; Paracetamol metabolism; Pharmacokinetics; Pharmacology. Drug treatments; Reference Standards; Saliva - chemistry; Assay; Paracetamol metabolism; Pharmacokinetics; Liver disease; Human; Urine; Metabolite; HPLC chromatography; Hepatic disease; Blood plasma; Chronic; Liver failure; Analgesic; Antipyretic; Digestive diseases; Child; Saliva; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/0731-7085(95)01303-3

A high-performance liquid chromatographic (HPLC) assay of paracetamol and its metabolites is described. The method for quantifying the metabolism of paracetamol in children with chronic liver disease and the good correlation between plasma and salivary concentrations of paracetamol is demonstrated. Despite an increasing bias between the two methods with increasing concentration of paracetamol, it is concluded that salivary assay is satisfactory for characterising paracetamol pharmacokinetics in the group of patients studied.