Velocity of axonal transport of phospholipid in rat sciatic nerve

• Published in: Experimental neurology Vol. 82; no. 3; pp. 706 - 710
• Main Authors: Guy, J.R., Bisby, M.A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.01.1983; Elsevier
• Subjects: Animals; Axonal Transport; Biological and medical sciences; Cold Temperature; Fundamental and applied biological sciences. Psychology; Hot Temperature; Isolated neuron and nerve. Neuroglia; Male; Phospholipids - metabolism; Rats; Rats, Inbred Strains; Sciatic Nerve - metabolism; Vertebrates: nervous system and sense organs; [ 3H]choline; TCA; Vertebrata; Mammalia; Rat; Axoplasmic transport; Rodentia; Phospholipid; Sensorimotor nerve
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1016/0014-4886(83)90092-4

The difficulties involved in measuring axonal transport velocity for phospholipid in mammalian axons have been overcome by using a reversible local cold-block technique which causes a local accumulation of labeled phospholipid in rat sciatic nerve that follows injection of [methyl 3H]choline chloride into the L5 dorsal root ganglion. On rewarming the nerve a distinct front of [ 3H]-activity moves distally from the cold-block site at 17.8 mm·h −1, a velocity not significantly different from that previously determined for protein. This observation provides further support for the hypothesis that fast axonal transport conveys membranes assembled in the cell body.