Pseudomonas aeruginosa lasA gene: determination of the transcription start point and analysis of the promoter/regulatory region
The elastolytic activity of the opportunistic pathogen Pseudomonas aeruginosa is due to the combined activities of at least three secreted proteins: elastase, LasA and alkaline protease. Transcription of both the lasA gene and the elastase structural gene, lasB, requires the transcriptional activato...
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Published in | Gene Vol. 129; no. 1; pp. 113 - 117 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
15.07.1993
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | The elastolytic activity of the opportunistic pathogen
Pseudomonas aeruginosa is due to the combined activities of at least three secreted proteins: elastase, LasA and alkaline protease. Transcription of both the
lasA gene and the elastase structural gene,
lasB, requires the transcriptional activator LasR. In order to localize the promoter elements involved in
lasA expression, the transcription start point (
tsp) for
lasA was localized by S1 protection and primer extension analysis. The DNA sequence of the region upstream from the
tsp was determined, and a putative σ
70 promoter was identified. Sequence comparison with the
lasB promoter region revealed two areas of considerable homology which could act as potential binding sites for LasR or other, as yet unidentified, regulatory proteins. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(93)90705-8 |