Calcification of cross-linked collagen-elastin membrane implants in vivo and their proposed use in bone regeneration

• Published in: Biomaterials Vol. 17; no. 15; pp. 1489 - 1497
• Main Authors: Vardaxis, N.J., Boon, M.E., Ruijgrok, J.M.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.08.1996; Elsevier Science
• Subjects: Animals; Biocompatible Materials; Biological and medical sciences; Bone and Bones - pathology; Bone and Bones - physiology; Bone and Bones - surgery; bone morphogenetic protein; Calcinosis; Collagen; cross-linking; dystrophic calcification; Elastin - analysis; elastin fibres; Glutaral; implantation; Inflammation; Male; Medical sciences; Microscopy, Confocal; microwave irradiation; Microwaves; Prostheses and Implants; Rats; Rats, Wistar; Regeneration; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Swine; Technology. Biomaterials. Equipments; Time Factors; dystrophic calcification; implantation; cross-linking; Collagen; elastin fibres; microwave irradiation; bone morphogenetic protein; Elastin; Artificial membrane; Bone remodeling; Experimental study; Physical properties; Pig; In vivo; Vertebrata; Mammalia; Histopathology; Surgical implantation; Animal; Calcification; Microwave irradiation; Biocompatibility; Biomaterial; Artiodactyla; Chemical properties; Glutaraldehyde tanning; Ungulata; Medical application; Comparative study
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/0142-9612(96)89773-0

Factors influencing the in vivo calcification of porcine collagen membranes containing elastic fibres were investigated by light and confocal microscopy. Two glutaraldehyde (GA) cross-linking protocols were used: a new one involving microwaving (NEWGA), and a conventional method using GA treatment at room temperature (OLDGA). We observed that the physical and chemical properties of implanted membranes will influence the acute inflammatory response, which initially checks the calcification process. The NEWGA membranes are superior in cases where rapid calcification is desired. In this context, it is proposed that NEWGA membranes may serve as a useful delivery system for bone morphogenetic protein (BMP).