Neuroimmunological electron microscopy with microwave-accelerated fixation

Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment....

Full description

Saved in:
Bibliographic Details
Published inJournal of immunological methods Vol. 110; no. 2; p. 267
Main Authors Armati, P J, Pollard, J D, Van Reyk, D, Van der Lubbe, L
Format Journal Article
LanguageEnglish
Published Netherlands 13.06.1988
Subjects
Aldehydes
Animals
Antigen-Antibody Complex - analysis
Endosomes - ultrastructure
Fixatives
Ganglia, Spinal
Glutaral
Microscopy, Electron - methods
Microwaves
Rats
Rats, Inbred Strains
Schwann Cells - analysis
Schwann Cells - immunology
Schwann Cells - ultrastructure
Online AccessGet more information

Cover

More Information
Summary:Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment. Using 1.25% glutaraldehyde and microwave fixation ideal preservation and demonstration of MHC class I antigen on Schwann cells was obtained by the peroxidase method.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(88)90113-5