A simple method for making ion-selective microelectrodes suitable for intracellular recording in vertebrate cells

A simple procedure for manufacturing Cl-, K+, and pH liquid membrane ion-sensitive microelectrodes is presented in detail. Electrodes suitable for recording from the specimen of interest are back-filled with a small amount of silane solution and heated for 5 min on a hot plate at a temperature betwe...

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Bibliographic Details
Published inJournal of neuroscience methods Vol. 15; no. 2; p. 141
Main Authors Borrelli, M J, Carlini, W G, Dewey, W C, Ransom, B R
Format Journal Article
LanguageEnglish
Published Netherlands 01.10.1985
Subjects
Animals
Cell Physiological Phenomena
In Vitro Techniques
Microelectrodes
Neurophysiology - instrumentation
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Summary:A simple procedure for manufacturing Cl-, K+, and pH liquid membrane ion-sensitive microelectrodes is presented in detail. Electrodes suitable for recording from the specimen of interest are back-filled with a small amount of silane solution and heated for 5 min on a hot plate at a temperature between 400 and 500 degrees C, after which they are injected with the ion-sensitive resin. The procedure is adaptable to many different glass stocks, e.g., single-barreled, double-barreled, or theta glass, and can be used to produce electrodes having a wide range of tip sizes for recording either extracellular or intracellular ion activities. Another advantage of the method is speed; up to 10 electrodes can be prepared simultaneously, permitting over 40 functional electrodes to be made per hour.
ISSN:0165-0270
DOI:10.1016/0165-0270(85)90051-2