A Sexually Transmitted Disease (STD) DNA chip for the diagnosis of genitourinary infections

• Published in: Biosensors & bioelectronics Vol. 26; no. 11; pp. 4314 - 4319
• Main Authors: Chung, Won-young, Jung, Ye Lim, Park, Ki Soo, Jung, Cheulhee, Shin, Sung Chul, Hwang, Sang-Joon, Cho, Dae-Yeon, Cha, Sun Ho, Lim, Si Kyu, Park, Hyun Gyu
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.07.2011; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; DNA chip; Equipment Design; Female; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Genitourinary infection; Gold; Humans; Male; Metal Nanoparticles; Multiplex PCR; Oligonucleotide Array Sequence Analysis - instrumentation; Oligonucleotide Array Sequence Analysis - methods; Polymerase Chain Reaction; Republic of Korea; Sexually Transmitted Diseases - diagnosis; Sexually Transmitted Diseases - microbiology; Sexually Transmitted Diseases - parasitology; Sexually Transmitted Diseases - virology; STD pathogen; Republic of Korea; STD pathogen; Multiplex PCR; Genitourinary infection; DNA chip; Infection; Polymerase chain reaction; Disease; Pathogenic; Diagnosis
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2011.04.024

The results of an investigation aimed at the development of a DNA chip for the detection of genitourinary infections are described. Through analysis of over 35,000 clinical cases, 14 pathogens which are most abundantly found among Koreans were selected and candidate sequences for capture probes were accordingly chosen by considering their sequences and β-globin house-keeping gene. Among this group, the most suitable capture probe sequences were selected by employing repeated chip tests in which they are immobilized on a glass chip by using a recently developed novel gold nanoparticles-based method. A multiplex PCR method was established to generate fluorescence-labeled sequences for all 14 pathogens along with the β-globin gene. By using optimized hybridization conditions, the final chip was constructed and employed to diagnose reliably both single and multiple infections in clinical human samples for 14 target pathogens. The results show that the novel chip methodology serves as a highly reliable and convenient tool for the diagnosis of Sexually Transmitted Diseases (STDs). Furthermore, this study has its great significance in that it demonstrates the entire process from statistical analysis of a large number of clinical cases to the final development of STD DNA chip just ready to be applied or commercialized in the clinical diagnostic field.