Effects of intratracheal administration of bleomycin on GSH-shuttle enzymes, catalase, lipid peroxidation, and collagen content in the lungs of hamsters

• Published in: Toxicology and applied pharmacology Vol. 71; no. 1; p. 132
• Main Authors: Giri, S N, Chen, Z L, Younker, W R, Schiedt, M J
• Format: Journal Article
• Language: English
• Published: United States 01.01.1983
• Subjects: Animals; Bleomycin - toxicity; Catalase - analysis; Collagen - analysis; Cricetinae; Glucose-6-Phosphatase - analysis; Glutathione Peroxidase - analysis; Glutathione Reductase - analysis; Lipid Peroxides - metabolism; Lung - drug effects; Lung - metabolism; Male; Mesocricetus; Superoxide Dismutase - analysis
• ISSN: 0041-008X
• DOI: 10.1016/0041-008X(83)90052-2

Intratracheal administration of one unit of bleomycin increased significantly the glucose-6-phosphate dehydrogenase activity to 147, 135, 163, 207, 278, and 148% of the control at 1, 2, 4, 7, 14, and 21 days post-treatment, respectively. The glutathione peroxidase activity was significantly increased to 147 and 124% of the control at 7 and 14 days post-treatment, respectively. The glutathione reductase and catalase activities were significantly elevated to 132, 174, and 145% of the control in the former and 160, 213, and 160% of the control in the latter case at 7, 14, and 21 days post-treatment, respectively. The amount of thiobarbituric acid reacting products as an index of lipid peroxidation was found to be significantly increased to 251, 248, and 253% of the control at 4, 7, and 14 days after treatment, respectively. The amount of total lung collagen in treated hamsters was significantly increased to 140, 197, and 245% of the control at 7, 14, and 21 days post-treatment, respectively. These findings indicate that the increases in the activities of antioxidant enzymes in lungs of bleomycin-treated hamsters result from the multiple cyto-dynamic events such as infiltration of inflammatory cells, and reparative and proliferative processes which generally follow lung injury caused by any oxidant.