Genetic diversity of colistin resistance Nosocomial Acinetobacter baumannii strains from Iran

• Published in: Journal of research in medical sciences Vol. 26; no. 1; p. 125
• Main Authors: Seyyedi, Maryam, Shapouri, Reza, Zeighami, Habib, Shokoohizadeh, Leili
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer - Medknow 01.01.2021; Wolters Kluwer Medknow Publications
• Subjects: acinetobacter baumannii; antibiotic resistance; molecular typing; Original; antibiotic resistance; Acinetobacter baumannii; molecular typing
• ISSN: 1735-1995; 1735-7136
• DOI: 10.4103/jrms.JRMS_1023_20

Drug-resistant is a global health problem since its ability to acquire new resistance mechanisms. Here, we aimed to determine the association of common types of and assess their drug resistance of and contribution of integrons ( ) and oxacillinase genes in Zanjan, Iran. Among 68 isolated Acinetobacters from patients, 48 isolates were strains. Antibiotic susceptibility pattern and colistin resistance were determined by disk diffusion and broth microdilution, respectively. The presence of , , , and oxacillinase genes examined using polymerase chain reaction. The clonal relationship of clinical isolates of determined by Pulsed Field Gel Electrophoresis method. The results showed the highest antibiotic susceptibility (58%) for colistin. 96% of isolates were considered as multidrug resistant, and 46% as extensively drug resistant, and 16% as pandrug resistant. Frequencies of , , resistance genes were 60%, 28%, and 0%, respectively, and 12% of strains had no isoform of Ints. Frequencies of Carbapenem resistance genes were 74%, 24%, 100%, and 4% for blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58, respectively. The above samples were group into 26 pulsotypes. The studied strains had several resistance genes, and the colistin resistance showed an extraordinary ascending tendency that could be a severe issue in nosocomial infections, and the presence of high genetic diversity indicated a variation in strains and possibly a variety of sources of contamination or infection.