Conserved DNA sequences in Chlamydial plasmids

• Published in: Plasmid Vol. 22; no. 2; pp. 91 - 98
• Main Authors: Hugall, Andrew, Timms, Peter, Girjes, Adeeb A., Lavin, Martin F.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1989; Elsevier
• Subjects: Animals; Bacteriology; Base Sequence; Biological and medical sciences; Cattle; Chlamydia - genetics; Chlamydia psittaci; Chlamydia trachomatis - genetics; Chlamydophila psittaci - genetics; Cloning, Molecular; DNA - genetics; Fundamental and applied biological sciences. Psychology; Genetics; Hybridization, Genetic; Microbiology; Molecular Sequence Data; Plasmids; Molecular hybridization; Chlamydiaceae; Nucleotide sequence; Enzyme; Chlamydia psittaci; Chlamydia trachomatis; Tandemly repeated sequence; Characterization; Plasmid; Conserved sequence; Restriction endonuclease; Cross reaction; Chlamydiales; Bacteria; Molecular cloning; Southern blotting
• ISSN: 0147-619X; 1095-9890
• DOI: 10.1016/0147-619X(89)90018-8

Two 7.4-kb plasmids from Chlamydia psittaci have been cloned and characterized. These plasmids are quite distinct from the 6.2-kb C. psittaci and the C. trachomatis plasmids when compared by restriction endonuclease analysis. The plasmids show considerable cross-hybridization, with only a small region highly conserved and identified as a 4 × 22-bp tandemly repeated region. This sequence is identical in the two size categories of C. psittaci plasmids and differs from C. trachomatis plasmids by only 2 bp in the 22-bp motif. AT-rich clusters 5′ to the repeat region which are present in C. trachomatis and Escherichia coli plasmids were absent from both classes of C. psittaci plasmids. Extensive regions are less highly conserved but show a sufficient degree of cross-hybridization to suggest that the plasmids are homologous.