A lectin-binding immunoassay indicates a possible glycosylated growth hormone in the human pituitary gland

• Published in: Biochemical and biophysical research communications Vol. 140; no. 2; pp. 491 - 497
• Main Authors: Sinha, Y.N., Lewis, U.J.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 30.10.1986; Elsevier
• Subjects: Applied sciences; Binding, Competitive; Concanavalin A; Exact sciences and technology; Growth Hormone - analogs & derivatives; Growth Hormone - analysis; Humans; Immunologic Techniques; Other techniques and industries; Pituitary Gland - analysis; Radioimmunoassay - methods; hGH-N; PBS; GH; BSA; SDS-PAGE; Con A; hGH; hGH-V; G-PRL; PRL; RIA; G-hGH
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(86)90759-X

By means of a lectin binding immunoassay we detected a glycosylated human growth hormone-immunoreactive substance in the human pituitary gland. The assay involves reacting the test substance with concanavalin A immobilized on a solid support followed by treatment with human growth hormone antibodies. Quantitation is achieved by reaction of the concanavalin A-glycoprotein-antibody complex with an 125I-labeled second antibody. Specificity of the assay for a glycosylated human growth hormone was indicated by inhibition of binding by N-acetyl-D-glucosamine. Furthermore, use of antiserum that had been absorbed with human growth hormone reduced the amount of radioactivity bound to the tubes. Western blotting of pituitary immunoprecipitates after gel electrophoresis in sodium dodecyl sulfate revealed human growth hormone-immunoreactive components that also reacted with concanavalin A.