Application of cell culture toxicity tests to the development of implantable biosensors

• Published in: Biosensors & bioelectronics Vol. 6; no. 8; pp. 653 - 661
• Main Authors: Zhang, Yanan, Bindra, Dilbir S., Barrau, Marie-Bernadette, Wilson, George S.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 1991; Elsevier Science
• Subjects: Agar; agar diffusion; biocompatibility; Biocompatible Materials; Biological and medical sciences; Biosensing Techniques; Biotechnology; cell culture; Cell Line; Equipment Design; Fundamental and applied biological sciences. Psychology; Glucose - analysis; glucose biosensor; Glutaral; L929 fibroblast; Methods. Procedures. Technologies; Polymers - isolation & purification; Polymers - toxicity; toxicity test; Various methods and equipments; L929 fibroblast; agar diffusion; toxicity test; cell culture; biocompatibility; glucose biosensor; Implanted material; Biocompatibility; Toxicity; Biosensor
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/0956-5663(91)87018-7

Cell culture toxicity testing methods were modified and applied to the development of implantable glucose microsensors, and positive and negative control materials suitable for the microsensor assessment were established. The location, source and degree of the toxic effect in a multi-component biosensor was spatially visualized with cell monolayers. A freshly prepared sensor showed moderate toxicity, mainly as a result of the presence of glutaraldehyde and the residual solvents in the polymer layers. However, it was possible to reduce the toxicity by removing the leachable toxic substances through extraction in phosphate, buffer, and a non-toxic sensor was readily obtained.