Developmental expression in the rat cerebellum of SC1, a putative brain extracellular matrix glycoprotein related to SPARC

• Published in: Brain research Vol. 633; no. 1; pp. 197 - 205
• Main Authors: Mendis, Duane B., Shahin, Susan, Gurd, James W., Brown, Ian R.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 07.01.1994; Amsterdam Elsevier; New York, NY
• Subjects: Activated-Leukocyte Cell Adhesion Molecule; Animals; Bergmann glia; Biochemistry and metabolism; Biological and medical sciences; Cell migration; Cell Movement; Central nervous system; Cerebellum - cytology; Cerebellum - growth & development; Cerebellum - metabolism; Extracellular Matrix Proteins - biosynthesis; Female; Fundamental and applied biological sciences. Psychology; Glycoproteins - biosynthesis; Immunohistochemistry; In Situ Hybridization; Nerve Tissue Proteins - biosynthesis; Neuroglia - metabolism; Neurons - physiology; Pregnancy; Rats; Rats, Wistar; RNA, Messenger - biosynthesis; Vertebrates: nervous system and sense organs; Bergmann glia; In situ hybridization; Cell migration; Cerebellum; Glial cell; Rat; Migration; Rodentia; Central nervous system; Cell cell interaction; Neuroglía; Glycoproteins; Gene expression; Postnatal development; Vertebrata; Mammalia; Neuron; Extracellular matrix; Brain (vertebrata)
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/0006-8993(94)91540-7

In the nervous system, extracellular matrix (ECM) molecules have been shown to have effects on cell migration, process outgrowth and the survival of neurons. Recently we have described the molecular cloning of SC1, a putative brain extracellular matrix glycoprotein, showing partial similarity to the ECM glycoprotein SPARC/osteonectin. We have now examined the expression of SC1 during the development of the rat cerebellum at both the protein and mRNA levels. Our results indicate that SC1 is both temporally and spatially regulated during this process. Bergmann glial cells express SC1 mRNA and the resultant protein is deposited along the length of their radial fibres during the process of granule cell migration in the developing cerebellum. SC1 mRNA and protein is also found in the adult cerebellum, concentrated in the Bergmann glial cells and their radial processes, indicating that this putative ECM molecule continues to play roles in the central nervous system after migration and proliferative events have ceased.