Adaptive response to radiation damage in human lymphocytes conditioned with hydrogen peroxide as measured by the cytokinesis-block micronucleus technique

• Published in: Mutation research Vol. 301; no. 2; pp. 135 - 141
• Main Authors: Domínguez, I., Panneerselvam, N., Escalza, P., Natarajan, A.T., Cortés, F.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.1993; Elsevier
• Subjects: Adaptation, Physiological; Antimutagenic Agents - pharmacology; Biological and medical sciences; Biological effects of radiation; Cell Cycle - drug effects; Chromosome Aberrations; Cytochalasin B - pharmacology; Cytokinesis-block micronucleus method; DNA Repair; Free Radicals - metabolism; Fundamental and applied biological sciences. Psychology; G2 Phase - radiation effects; Human lymphocytes; Humans; hydrogen peroxide; Hydrogen Peroxide - pharmacology; Hydrogen peroxide pre-exposure; Ionizing radiations; lymphocytes; Lymphocytes - radiation effects; man; micronuclei; Micronucleus Tests; Radiation Tolerance; Radiation-Protective Agents - pharmacology; Reactive Oxygen Species - metabolism; Tissues, organs and organisms biophysics; X radiation; Human lymphocytes; Hydrogen peroxide pre-exposure; Cytokinesis-block micronucleus method; Human; Cell culture; Radioprotector; Mutagenicity testing; Hydrogenperoxides; Pretreatment; X ray irradiation; Lymphocyte; Dose activity relation
• ISSN: 0165-7992; 0027-5107
• DOI: 10.1016/0165-7992(93)90036-U

We have made use of the cytokinesis-block micronucleus method to evaluate the adaptive response in human lymphocytes from two donors conditioned with low doses of hydrogen peroxide before irradiation with either 1.5 or 3.0 Gy of X-rays. A protective effect of pre-exposure to H 2O 2 against radiation damage detected as micronuclei in binucleate cells was evident in cells allowed to recover for different periods after X-ray exposure, though cells challenged with 3.0 Gy of X-rays showed the adaptive response at later fixation time than those exposed to the lower dose (1.5 Gy). We propose this protocol as an interesting alternative to the single fixation method to score chromosomal aberrations at metaphase for the study of the adaptive response.