Ionic dependence of adenosine uptake into cultured astrocytes

• Published in: Brain research Vol. 661; no. 1; pp. 1 - 8
• Main Authors: Bender, Alex S., Woodbury, Dixon M., Steve White, H.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 24.10.1994; Amsterdam Elsevier; New York, NY
• Subjects: Adenosine - metabolism; Adenosine uptake; Animals; Animals, Newborn; Astrocyte; Astrocytes - drug effects; Astrocytes - metabolism; Benzopyrans - pharmacology; Biological and medical sciences; Biological Transport; BRL 34915; Calcimycin - pharmacology; Calcium - pharmacology; Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology; Cells, Cultured; Cerebral Cortex - metabolism; Cromakalim; Fundamental and applied biological sciences. Psychology; Furosemide - pharmacology; Glyburide - pharmacology; Ion; Ionophore; Ionophores - pharmacology; Isolated neuron and nerve. Neuroglia; Kinetics; Mice; Niacinamide - analogs & derivatives; Niacinamide - pharmacology; Nicorandil; Nigericin - pharmacology; Omeprazole; Omeprazole - pharmacology; Potassium - pharmacology; Pyrroles - pharmacology; Sodium - pharmacology; Valinomycin - pharmacology; Vasodilator Agents - pharmacology; Vertebrates: nervous system and sense organs; Omeprazole; BRL 34915; Adenosine uptake; Ion; Astrocyte; Ionophore; Adenosine; Cerebral cortex; Rodentia; Central nervous system; Homeostasis; Ions; Neuroglía; Metabolism; In vitro; Uptake; Vertebrata; Mammalia; Sodium; Mouse; Membrane transport; Chlorides; Proton; Brain (vertebrata)
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/0006-8993(94)91173-8

Adenosine uptake in cultured astrocytes is dependent on various ions and energy metabolism. The Na +-gradient plays an important role, since nigericin, ouabain, amiloride and substitution of Na + with choline inhibited adenosine uptake. The proton-gradient was importance, since carbonycyanide m-chlorophenylhydrozone (CCCP) and omeprazole also inhibited adenosine uptake. Furthermore, adenosine uptake was dependent on Cl − anion. Substitution of Cl − with isethionate, as well as DIDS or furosemide inhibited adenosine uptake. Adenosine uptake was also sensitive to Ca 2+ gradient, removal of extracellular Ca 2+ and calcimycin inhibited adenosine uptake. Adenosine uptake was not dependent on extracellular K + and was not affected by valinomycin. Although, K +-channel openers (BRL 34195 and nicorandil) as well as the K +-channel antagonist, glyburide, inhibited adenosine uptake, the inhibitory effect of BRL 34915 was not antagonized by glyburide. Rotenone and 2,4-dinitrophenol also inhibited adenosine uptake. Ionic dependence and metabolic energy dependence of adenosine uptake suggest that uptake is primarily an active process.