Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals

• Published in: Journal of Chromatography A Vol. 1217; no. 16; pp. 2296 - 2305
• Main Authors: Rosa, P.A.J., Ferreira, I.F., Azevedo, A.M., Aires-Barros, M.R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.04.2010; Amsterdam; New York: Elsevier
• Subjects: Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - isolation & purification; Aqueous two-phase systems (ATPSs); Biopharmaceuticals; Biopharmaceutics - methods; Chemical Fractionation - methods; Chromatography, Liquid; Downstream processing; Drug Industry - methods; Manufacturing; Protein Biosynthesis; Proteins - isolation & purification; Water - chemistry; Aqueous two-phase systems (ATPSs); Manufacturing; Biopharmaceuticals; Downstream processing
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2009.11.034

The number of biotechnology-based pharmaceuticals in the late-stage pipeline has been increasing more than ever. As a result, there is an enhanced demand for more efficient and cost-effective processes. During the last years, the upstream technology for the production of biopharmaceuticals has been considerably improved. Continuous discoveries in molecular biology and genetics, combined with new advances in media and feed development, have significantly increased the production titres. In order to keep up this gain, it is now essential to design new, as well as to improve the existing downstream processes that remain an unresolved bottleneck. This review evaluates several alternatives to the currently established platforms for the downstream processing biopharmaceuticals, with main focus on aqueous two-phase extraction.