Exploration of the Role of Serine Proteinase Inhibitor A3 in Alcohol Dependence Using Gene Expression Omnibus Database

• Published in: Frontiers in psychiatry Vol. 12; p. 779143
• Main Authors: Zhang, Bo, Wang, Gang, Huang, Cheng Bing, Zhu, Jian Nan, Xue, Yong, Hu, Jian
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 12.01.2022
• Subjects: alcohol dependence; bioinformatics analysis; differently expressed genes; Psychiatry; relapse biomarkers; SERPINA3; SERPINA3; differently expressed genes; alcohol dependence; bioinformatics analysis; relapse biomarkers
• ISSN: 1664-0640; 1664-0640
• DOI: 10.3389/fpsyt.2021.779143

Alcohol dependence is an overall health-related challenge; however, the specific mechanisms underlying alcohol dependence remain unclear. Serine proteinase inhibitor A3 ( ) plays crucial roles in multiple human diseases; however, its role in alcohol dependence clinical practice has not been confirmed. We screened Gene Expression Omnibus (GEO) expression profiles, and identified differentially expressed genes (DEGs). Protein-protein interaction (PPI) networks were generated using STRING and Cytoscape, and the key clustering module was identified using the MCODE plugin. -based target microRNA prediction was performed using online databases. Functional enrichment analysis was performed. Fifty-eight patients with alcohol dependence and 20 healthy controls were recruited. Clinical variables were collected and follow-up was conducted for 8 months for relapse. was identified as a DEG. and miR-137 were identified after PPI analysis. The enriched functions and pathways included acute inflammatory response, response to stress, immune response, and terpenoid backbone biosynthesis. concentrations were significantly elevated in the alcohol dependence group than in healthy controls ( < 0.001). According to the median value of expression level in alcohol dependence group, patients were divided into high (≥2677.33 pg/ml, = 29) and low groups (<2677.33 pg/ml, = 29). Binary logistic analysis indicated that IL-6 was statistically significant ( = 0.015) Kaplan-Meier survival analysis did not indicate any difference in event-free survival between patients with low and high levels ( = 0.489) after 8 months of follow-up. Receiver characteristic curve analysis revealed that had an area under the curve of 0.921 ( < 0.0001), with a sensitivity and specificity of 93.1 and 80.0%, respectively. Cox regression analysis revealed that aspartate transaminase level was a negative predictor of relapse (β = 0.003; hazard ratio = 1.003; = 0.03). level was remarkably elevated in patients with alcohol dependence than healthy controls, indicating that is correlated with alcohol dependence. However, may not be a potential predictive marker of relapse with patients in alcohol dependence.