HeLa cells contain the atrial natriuretic peptide receptor with guanylate cyclase activity
Receptors for atrial natriuretic peptide (ANP) are heterogeneous: an ≈140-kDa receptor exhibits ANP-stimulated guanylate cyclase activity whereas an ≈65-kDa receptor is thought to act only as a clearancestorage protein. We have used photoaffinity labeling techniques to show that the human cell line,...
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Published in | Biochemical and biophysical research communications Vol. 164; no. 2; pp. 671 - 677 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
31.10.1989
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Receptors for atrial natriuretic peptide (ANP) are heterogeneous: an ≈140-kDa receptor exhibits ANP-stimulated guanylate cyclase activity whereas an ≈65-kDa receptor is thought to act only as a clearancestorage protein. We have used photoaffinity labeling techniques to show that the human cell line, HeLa, contains predominantly the ≈140-kDa ANP receptor. In contrast, several other cell lines contain primarily the ≈65-kDa receptor. In HeLa cells, ANP bound specifically to high affinity binding sites (Kd≈2 nM) and stimulated a rapid, dose-dependent accumulation of cGMP. These cell lines can thus provide useful models to study the multiple mechanisms of ANP action. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/0006-291X(89)91512-X |