Sickle cell anemia induces changes in peripheral lymphocytes E-NTPDase/E-ADA activities and cytokines secretion in patients under treatment

• Published in: Biomedicine & pharmacotherapy Vol. 73; pp. 102 - 108
• Main Authors: Castilhos, Lívia G, Doleski, Pedro H, Bertoldo, Tatiana M.D, Passos, Daniela F, Bertoncheli, Claudia de M, Rezer, João F.P, Schlemmer, Josiane B, Leal, Daniela B.R
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.07.2015
• Subjects: Adenosine Deaminase - blood; Adenosine Triphosphatases - blood; Adolescent; Adult; Anemia, Sickle Cell - blood; Anemia, Sickle Cell - enzymology; Anemia, Sickle Cell - therapy; Case-Control Studies; Cytokines; Cytokines - blood; E-ADA; E-NTPDase; Female; Humans; Internal Medicine; Lymphocytes - enzymology; Male; Medical Education; Middle Aged; Sickle cell anemia; Treatment Outcome; Young Adult; E-NTPDase; Sickle cell anemia; Cytokines; E-ADA
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2015.05.011

Abstract Sickle cell anemia (SCA) is characterized by hemoglobin polymerization that results in sickle-shaped red blood cells. The vascular obstruction by sickle erythrocytes is often inflammatory, and purinergic system ecto-enzymes play an important role in modulating the inflammatory and immune response. This study aimed to evaluate the E-NTPDase and E-ADA activities in lymphocytes of SCA treated patients, as well as verify the cytokine profile in this population. Fifteen SCA treated patients and 30 health subjects (control group) were selected. The peripheral lymphocytes were isolated and E-NTPDase and E-ADA activities were determined. Serum was separated from clot formation for the cytokines quantification. E-NTPDase (ATP and ADP as substrate) and E-ADA (adenosine as substrate) activities were increased in lymphocytes from SCA patients ( P < 0.001). The TNF-α and IL-6 serum cytokines showed decreased on SCA patients comparing to control ( P < 0.001). The regulation of extracellular nucleotides released in response to hypoxia and inflammation through E-NTPDase and E-ADA enzymes represent an important control of purine-mediated in the SCA disease, avoiding elevated adenosine levels in the extracellular medium and consequent organ injuries in these patients. The pro-inflammatory cytokines decreased levels by use of hydroxyurea occur in attempt to reduce the pro-inflammatory response and prevent vaso-oclusive crisis.