Single-Particle Tracking Shows that a Point Mutation in the Carnivore Parvovirus Capsid Switches Binding between Host-Specific Transferrin Receptors

Determining how viruses infect new hosts via receptor-binding mechanisms is important for understanding virus emergence. We studied the binding kinetics of canine parvovirus (CPV) variants isolated from raccoons-a newly recognized CPV host-to different carnivore transferrin receptors (TfRs) using si...

Full description

Saved in:
Bibliographic Details
Published inJournal of virology Vol. 90; no. 9; pp. 4849 - 4853
Main Authors Lee, Donald W, Allison, Andrew B, Bacon, Kaitlyn B, Parrish, Colin R, Daniel, Susan
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.05.2016
Subjects
Animals
Canine parvovirus
Capsid Proteins - chemistry
Capsid Proteins - genetics
Capsid Proteins - metabolism
Cell Line
Dogs
Kinetics
Microscopy, Fluorescence - methods
Molecular Imaging - methods
Parvovirus
Parvovirus, Canine - physiology
Point Mutation
Protein Binding
Raccoons
Receptors, Transferrin - metabolism
Spotlight
Staining and Labeling
Viral Tropism
Virus-Cell Interactions
Online AccessGet full text

Cover

More Information
Summary:Determining how viruses infect new hosts via receptor-binding mechanisms is important for understanding virus emergence. We studied the binding kinetics of canine parvovirus (CPV) variants isolated from raccoons-a newly recognized CPV host-to different carnivore transferrin receptors (TfRs) using single-particle tracking. Our data suggest that CPV may utilize adhesion-strengthening mechanisms during TfR binding and that a single mutation in the viral capsid at VP2 position 300 can profoundly alter receptor binding and infectivity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
D.W.L. and A.B.A. contributed equally to this work.
Citation Lee DW, Allison AB, Bacon KB, Parrish CR, Daniel S. 2016. Single-particle tracking shows that a point mutation in the carnivore parvovirus capsid switches binding between host-specific transferrin receptors. J Virol 90:4849–4853. doi:10.1128/JVI.03204-15.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.03204-15