Mass measurements of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate in a neuronal cell line stimulated with bradykinin: Inositolphosphate response shows desensitization

• Published in: Biochemical and biophysical research communications Vol. 181; no. 3; pp. 997 - 1003
• Main Authors: Donié, Frédéric, Reiser, Georg
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 31.12.1991; Elsevier
• Subjects: Animals; Biological and medical sciences; bradykinin; Bradykinin - pharmacology; Calcium - pharmacology; Cell Line; Cell physiology; correlation; desensitization; Fundamental and applied biological sciences. Psychology; Glioma; Hybrid Cells - drug effects; Hybrid Cells - metabolism; inositol 1,3,4,5-tetrakisphosphate; inositol 1,4,5-trisphosphate; Inositol 1,4,5-Trisphosphate - metabolism; Inositol Phosphates - metabolism; Kinetics; measurements; Mice; Molecular and cellular biology; Neuroblastoma; neurons; Neurotransmission; stimulation; InsP 3; InsP 4; EDTA; cyclic GMP; EGTA; Binding; Signal transduction; Stimulation; Desensitization; Cell line; Bradykinin
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(91)92035-I

In a neuronal cell line (108CC15, NG108-15) the levels of inositol 1,4,5-trisphosphate (InsP 3) and inositol 1,3,4,5-tetrakisphosphate (InsP 4), as measured by receptor binding assays, rise transiently after stimulation with bradykinin (EC 50 approx. 150 nM). Maximal InsP 3 level 354 pmol/mg protein (15-fold basal level) is obtained at 10 – 15 s after addition of bradykinin, the InsP 4 level rises maximally to 78 pmol/mg protein (14-fold basal level) at 20 – 30 s. In a rat glioma cell line, bradykinin (2 μM) causes a fast 6-fold increase in InsP 3 and InsP 4 levels. In the neuronal cells the bradykinin-dependent rise of the inositolphosphate levels is diminished with reduced extracellular Ca 2+ concentration. However, depletion of internal Ca 2+ stores does not affect the bradykinin-induced rise in InsP 3 and InsP 4 levels. Homologous desensitization to bradykinin occurs in the signal transduction pathway already at the production of inositolphosphates, since after a 2 min stimulation with bradykinin the rise in cellular masses of InsP 3 and InsP 4, inducible by a following second bradykinin stimulus,is substantially reduced.