Purification and crystallization of thymidine kinase from herpes simplex virus type 1

Thymidine kinase from herpes simplex virus type 1 (ATP: thymidine 5′-phosphotransferase; EC 2.7.1.21) has been purified from an overexpression system and crystallized against ammonium sulfate by using the hanging-drop technique. The tetragonal crystals are of space group P4 122 or P4 322, and have u...

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Published inJournal of molecular biology Vol. 202; no. 4; pp. 917 - 919
Main Authors Sanderson, M.R., Freemont, P.S., Murthy, H.M.K., Krane, J.F., Summers, W.C., Steitz, T.A.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 20.08.1988
Elsevier
Subjects
Biological and medical sciences
Crystalline structure
Crystallization
Fundamental and applied biological sciences. Psychology
herpes simplex virus 1
Molecular biophysics
Simplexvirus - enzymology
Structure in molecular biology
Thymidine Kinase - isolation & purification
X-Ray Diffraction
herpes simplex virus
HSV
thymidine kinase
TK
Virus
Thymidine kinase
Purification
Herpesvirus hominis 1
Herpesviridae
Alphaherpesvirinae
Crystallization
Crystalline structure
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Summary:Thymidine kinase from herpes simplex virus type 1 (ATP: thymidine 5′-phosphotransferase; EC 2.7.1.21) has been purified from an overexpression system and crystallized against ammonium sulfate by using the hanging-drop technique. The tetragonal crystals are of space group P4 122 or P4 322, and have unit cell dimensions a = b = 84 A ̊ , c = 180 A ̊ .
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(88)90569-4