Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis
The gene ( azu) encoding azurin from Alcaligenes denitrificans has been cloned and sequenced. The gene codes for a pre-protein with a 19-aa signal peptide. Comparison with the sequences coding for the blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis reveals the presence of n...
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Published in | Gene Vol. 90; no. 1; pp. 15 - 20 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
31.05.1990
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | The gene (
azu) encoding azurin from
Alcaligenes denitrificans has been cloned and sequenced. The gene codes for a pre-protein with a 19-aa signal peptide. Comparison with the sequences coding for the blue copper proteins from
Pseudomonas aeruginosa and
Alcaligenes faecalis reveals the presence of
ntrA and
fnr boxes in front of all three genes, instead of a regular [−10, −35]-promoter. In
P. aeruginosa, the
azu gene is terminated by a bidirectional terminator and flanked by open reading frames on the opposite strand. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(90)90434-S |