Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis

• Published in: Gene Vol. 90; no. 1; pp. 15 - 20
• Main Authors: Hoitink, Carla W.G., Woudt, Lambertus P., Turenhout, Jack C.M., van de Kamp, Mart, Canters, Gerard W.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 31.05.1990; Amsterdam Elsevier; New York, NY
• Subjects: Alcaligenes - genetics; Alcaligenes denitrificans; Alcaligenes faecalis; Amino Acid Sequence; anaerobic regulation; Azurin - genetics; Bacterial Proteins - genetics; Base Sequence; bidirectional terminator; Biological and medical sciences; Cloning, Molecular; fnr box; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; ntrA box; plastocyanins; Promoter Regions, Genetic; promoters; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Recombinant DNA; Regulatory Sequences, Nucleic Acid; Restriction Mapping; signal peptide; ntrA box; A; ORF; P.a; nt; Recombinant DNA; bp; N; Ap; tsp; SD; fnr box; R; kb; Y; promoters; aa; bidirectional terminator; RBS; SSC; anaerobic regulation; signal peptide; XGal; LB; A.d; IPTG; A.f; Azu; plastocyanins; Leader peptide; Azurin; Nucleotide sequence; Transcription promoter; DNA; Bacteria; Homology; Molecular cloning; Cuproprotein
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(90)90434-S

The gene ( azu) encoding azurin from Alcaligenes denitrificans has been cloned and sequenced. The gene codes for a pre-protein with a 19-aa signal peptide. Comparison with the sequences coding for the blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis reveals the presence of ntrA and fnr boxes in front of all three genes, instead of a regular [−10, −35]-promoter. In P. aeruginosa, the azu gene is terminated by a bidirectional terminator and flanked by open reading frames on the opposite strand.