Kinetic comparison of procaspase-3 and caspase-3

• Published in: Archives of biochemistry and biophysics Vol. 442; no. 1; pp. 125 - 132
• Main Authors: Karki, Pratap, Lee, Jungsup, Shin, Song Yub, Cho, Byungyun, Park, Il-Seon
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2005
• Subjects: Binding Sites; Caspase 3; Caspases - genetics; Caspases - metabolism; Catalysis; Crystallography, X-Ray; Cysteine; Cysteine - chemistry; DEPC; Diethyl Pyrocarbonate - metabolism; Enzyme Activation; Enzyme Precursors - genetics; Enzyme Precursors - metabolism; Histidine; Histidine - chemistry; Hydrogen-Ion Concentration; IAM; Inactivation; Iodoacetamide - chemistry; Kinetics; Mutation; p Ka; Procaspase-3; Cysteine; Histidine; IAM; Procaspase-3; p K a; Kinetics; DEPC; Inactivation; Caspase-3
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2005.07.023

Caspases, the key enzymes in apoptosis, are synthesized as proenzymes and converted into active form by proteolytic cleavage. The residues on active site reorganize during the activation process as shown in the comparative studies of crystallographic structures of procaspase-7 and its mature form. On the other hand, the proenzyme itself has some activity. Aiming to characterize the activation process, the comparative kinetic study for the pro- and mature caspase-3 was performed. In 1/ K M versus pH study, a residue with p K a of 6.89 ± 0.13 was detected only in caspase-3. While V max versus pH kinetic results were consistent with the existence of a residue with p K a of 6.21 ± 0.06 in procaspase-3 mutant (D9A/D28A/D175A) but not in caspase-3. In the inactivation assays with diethylpyrocarbonate, a residue (p K a, 6.61 ± 0.05) could be determined only for caspase-3 whereas with iodoacetamide a residue with p K a value (6.01 ± 0.05) could be assigned only for procaspase-3. Considering that those residues could be protected by caspase-3-specific inhibitor from the inactivation, the modifiers are histidine- and cysteine-specific, respectively, and the involvement of these residues in the characteristic catalytic dyad of caspases, the results indicate that the p K a values of the catalytic histidine and cysteine residues are changed during the activation process.