Magnetic lipase active in organic solvents

• Published in: Biochemical and biophysical research communications Vol. 142; no. 2; pp. 291 - 296
• Main Authors: Takahashi, Katsunobu, Tamaura, Yutaka, Kodera, Yoh, Mihama, Takeshi, Saito, Yuji, Inada, Yuji
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 30.01.1987; Elsevier
• Subjects: Applied sciences; Benzene; Exact sciences and technology; Lipase - analysis; Magnetics; organic compounds; Other techniques and industries; Particle Size; Polyethylene Glycols - pharmacology; Solvents; triacylglycerol lipase
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(87)90271-3

Magnetic lipase (magnetite particles coated with polyethylene glycol-modified lipase) was prepared in two steps: (1)Lipase was coupled with 2,4-bis(O-methoxypolyethylene glycol)-6-chloro-s-triazine, activated PEG 2, to obtain polyethylene glycol-modified lipase, PEG-lipase. (2)The PEG-lipase was added to the solution of ferrous(Fe 2+)- and ferric(Fe 3+)-ions with the pH value adjusted to 8.0–8.5 to obtain magnetic lipase. The magnetic lipase was dispersed in organic solvents such as benzene and 1,1,1-trichloroethane with the particle size of 120 ± 60 nm. The colloidal solution was very stable and no aggregation occured even after 5 days. A high enzymic activity (11.6 μmol/min/mg protein) for lauryl laurate synthesis was observed in 1,1,1-trichloroethane. The magnetic lipase was readily recovered from the organic solvents in a magnetic field of 6000 Oe without loss of the enzymic activity.