Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile

Genes annotated as and in the genome of serovar Typhimurium encode proteins homologous to cell division factors ZapA and ZapB, respectively. ZapA and ZapB mutants of are bile-sensitive. The amount of mRNA increases in the presence of a sublethal concentration of sodium deoxycholate (DOC) while mRNA...

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Published inFrontiers in microbiology Vol. 12; p. 647305
Main Authors Fernández-Fernández, Rocío, Hernández, Sara B, Puerta-Fernández, Elena, Sánchez-Romero, María A, Urdaneta, Verónica, Casadesús, Josep
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 25.02.2021
Subjects
bile resistance
Lon protease
MicA sRNA
Microbiology
Salmonella
Z-ring
Z-ring
bile resistance
Salmonella
Lon protease
MicA sRNA
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Summary:Genes annotated as and in the genome of serovar Typhimurium encode proteins homologous to cell division factors ZapA and ZapB, respectively. ZapA and ZapB mutants of are bile-sensitive. The amount of mRNA increases in the presence of a sublethal concentration of sodium deoxycholate (DOC) while mRNA remains unaffected. Increased mRNA level in the presence of DOC is not caused by upregulation of transcription but by increased stability of mRNA. This increase is suppressed by an mutation, suggesting the involvement of a small regulatory RNA. We provide evidence that such sRNA is MicA. The ZapB protein is degraded in the presence of DOC, and degradation appears to involve the Lon protease. We propose that increased stability of mRNA in the presence of DOC may counter degradation of bile-damaged ZapB, thereby providing sufficient level of functional ZapB protein to permit Z-ring assembly in the presence of bile.
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Edited by: Axel Cloeckaert, Institut National de Recherche pour l’agriculture, l’alimentation et l’environnement (INRAE), France
Verónica Urdaneta, Department of Infectious Diseases, Yale School of Medicine, The Anlyan Center, New Haven, CT, United States
These authors have contributed equally to this work
Present address: Sara B. Hernández, Department of Molecular Biology, Umeå University, Umeå, Sweden
María A. Sánchez-Romero, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain
Reviewed by: Juan A. Ayala, Autonomous University of Madrid, Spain; Laurent Aussel, Aix-Marseille Université, France
This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology
Elena Puerta-Fernández, Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS, CSIC), Sevilla, Spain
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.647305