Genetic variation at the STR loci D12S391 and CSF1PO in four populations from Austria, Italy, Egypt and Yemen

• Published in: Forensic science international Vol. 97; no. 1; pp. 37 - 45
• Main Authors: Klintschar, Michael, Ricci, Ugo, Al Hammadi, Nabil, Reichenpfader, Barbara, Ebner, Alexander, Giovannucci Uzielli, Maria Luisa
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ireland Ltd 12.10.1998; Elsevier; Elsevier Limited
• Subjects: Alleles; Austria; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Cloning; CSF1PO; D12S391; Egypt; Electrophoresis; Fundamental and applied biological sciences. Psychology; Genetic diversity; Genetic Variation; Genetics of eukaryotes. Biological and molecular evolution; Genetics, Population; Genotype; Genotype & phenotype; Human; Humans; Italy; Linkage disequilibrium; Paternity; Polymerase Chain Reaction; Population study; Populations; Short tandem repeats; Software; Tandem Repeat Sequences - genetics; Yemen; Austria; Asia; Europe; Africa; Egypt; Italy; Yemen; D12S391; Austria; Egypt; Italy; CSF1PO; Population study; Yemen; Human; Genetic variability; Genetic marker; Microsatellite DNA; Genetic identification; Forensic aspect; Population genetics; Polymorphism
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/S0379-0738(98)00130-3

The short tandem repeat systems (STRs) D12S391 and CSF1P0 were amplified by the polymerase chain reaction (PCR) on blood samples from 100 to 158 unrelated Austrians, Italians, Yemenians and Egyptians. The samples were analyzed by both native and denaturing electrophoresis and two primer pairs were tested for the CSF1PO locus. Except for the CSF1PO data on the Egyptians, no deviations from the Hardy–Weinberg equilibrium were detected. For D12S391, no significant differences were found between the two Arab populations and between the two European populations, but the differences between both Arab populations and the Italians were significant. For CSF1PO, differences were only observed between the Yemenians and all three other populations. No evidence of linkage disequilibrium between the two STRs was found. The observation of a D12S391 allele consisting of only 14 repeats was confirmed by sequencing.