The impact of the IL-1β, IL-1Ra, IL-2, IL-6 and IL-10 gene polymorphisms on the development of Behcet's disease and their association with the phenotype

• Published in: Medicina clinica Vol. 146; no. 9; p. 379
• Main Authors: Barış, Savaş, Akyürek, Ömer, Dursun, Ahmet, Akyol, Mesut
• Format: Journal Article
• Language: English
• Published: Spain 06.05.2016
• Subjects: Adolescent; Adult; Behcet Syndrome - diagnosis; Behcet Syndrome - genetics; Behcet Syndrome - therapy; Case-Control Studies; Female; Follow-Up Studies; Genetic Markers; Genetic Predisposition to Disease; Genotype; Humans; Interleukin 1 Receptor Antagonist Protein - genetics; Interleukin-10 - genetics; Interleukin-1beta - genetics; Interleukin-2 - genetics; Interleukin-6 - genetics; Interleukins - genetics; Male; Middle Aged; Phenotype; Polymorphism, Single Nucleotide; Prognosis; Young Adult; IL-1Ra; Enfermedad de Behcet; IL-6 and IL-10; Behcet's disease; IL-6 e IL-10; IL-2; IL-1β
• ISSN: 1578-8989
• DOI: 10.1016/j.medcli.2015.09.017

This trial was designed to investigate the effects of the interleukin (IL)-1β, IL-1Ra, IL-2, IL-6, IL-10 gene polymorphisms on Behcet's disease (BD) occurrence and the association between the polymorphisms and the phenotype. The study population consisted of 71 patients and 70 age and gender-matched healthy subjects. Each of the participants had 2cc of blood withdrawn, which was placed into a whole blood tube, and the DNA was obtained using the NucleoSpin(®) Blood DNA Isolation kit. To display the band lengths, the products were amplified using the primary pairs of the interleukins investigated and developed in a 2% agarose gel. There were no significant differences between the groups with respect to the IL-1Ra, IL-1β, IL-2, IL-6 and the IL-10 gene polymorphism distributions. In the patient group the IL-1RN2 gene polymorphism was detected to be statistically correlated with the presence of articular involvement (p=0.0283) and the IL-1β gene polymorphism was statistically correlated with the presence of an ocular lesion (p=0.0178). The evaluation of the IL-2 gene polymorphism (p=0.0065) and IL-10 gene polymorphism (p=0.0483) distributions with respect to age of BD onset revealed a statistically significant distribution. The statistical correlations between the articular involvement and IL-1RN, the ocular involvement and the IL-1β, and the age of disease onset and the IL-2 and IL-10 gene polymorphisms, detected for the first time in the literature, suggest that these polymorphisms could be statistically associated with the disease symptoms and used as prognostic factors.