Protein phosphorylation in the blood-brain barrier. Possible presence of marcks in brain microvessels

• Published in: Neurochemistry international Vol. 28; no. 1; pp. 59 - 65
• Main Authors: Catalán, R.Edgardo, Martínez, Ana M., Aragonés, M.Dolores, Hernandez, Félix
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1996; Elsevier
• Subjects: Alkaloids - pharmacology; Animals; Autoradiography; Biological and medical sciences; Blood-Brain Barrier - drug effects; Blood-Brain Barrier - physiology; Brain - blood supply; Capillaries - drug effects; Capillaries - metabolism; Capillaries - physiology; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Cerebrovascular Circulation - physiology; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Intracellular Signaling Peptides and Proteins; Male; Membrane Proteins; Molecular Weight; Myristoylated Alanine-Rich C Kinase Substrate; Nerve Tissue Proteins - metabolism; Phosphoproteins - metabolism; Phosphorus Radioisotopes; Phosphorylation; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Proteins - metabolism; Proteins - physiology; Rats; Rats, Wistar; Staurosporine; Tetradecanoylphorbol Acetate - pharmacology; Vertebrates: nervous system and sense organs; Proteins; Microcirculation; Vertebrata; Phosphorylation; Mammalia; Rat; Myristoylated alanine rich C kinase substrate protein; Rodentia; Central nervous system; Blood brain barrier; Brain (vertebrata)
• ISSN: 0197-0186; 1872-9754
• DOI: 10.1016/0197-0186(95)00060-L

The protein phosphorylation in rat brain microvessels has been examined; the major phosphorylated proteins correspond to a doublet of molecular weight 134–141 kDa, and four proteins of approx. 25, 55, 80 and 200 kDa. TPA (12- O-tetradecanoylphorbol-13-acetate) enhanced, in a few minutes, the phosphorylation of three major protein substrates with apparent molecular weights of 17.5, 44.5 and 80 kDa. These effects are inhibited by staurosporine. The 80 kDa protein resulted to be myristoylated alanine-rich C kinase substrate (MARCKS). This work demonstrates that protein kinase C plays an important role in protein phosphorylation in blood-brain barrier (BBB).