MiR-98-3p alleviates lipopolysaccharide-induced pulmonary microvascular endothelial barrier dysfunction by targeting DKK3 in sepsis-induced acute lung injury

• Published in: Journal of toxicological sciences Vol. 49; no. 7; pp. 289 - 299
• Main Authors: Zhong, Dan, Luo, Cong, Wang, Neng, Lin, Jie
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Society of Toxicology 2024; Japan Science and Technology Agency
• Subjects: Acute lung injury; Acute Lung Injury - etiology; Acute Lung Injury - genetics; Acute Lung Injury - metabolism; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Angiogenesis; Assaying; Capillary Permeability - drug effects; Cells, Cultured; DKK3; Endothelial barrier injury; Endothelial cells; Endothelial Cells - metabolism; Humans; Injuries; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Lipopolysaccharides; Lung - blood supply; Lungs; Membrane permeability; MicroRNAs - genetics; MicroRNAs - metabolism; Microvasculature; miR-98-3p; Pathogenesis; Permeability; Proteins; Rhodamine; Sepsis; Sepsis - complications; Sepsis - metabolism; Tight junctions; Tight Junctions - metabolism; Western blotting; Endothelial barrier injury; Acute lung injury; Angiogenesis; miR-98-3p; DKK3
• ISSN: 0388-1350; 1880-3989; 1880-3989
• DOI: 10.2131/jts.49.289

Background: Endothelial barrier dysfunction is critical for the pathogenesis of sepsis-induced acute lung injury (ALI). Lipopolysaccharide (LPS)-stimulated human pulmonary microvascular endothelial cells (HPMECs) are widely used as the cell model of sepsis-associated ALI for exploration of endothelial barrier dysfunction. Dickkopf (DKK) family proteins were reported to mediate endothelial functions in various diseases. The present study explored the effect of Dickkopf-3 (DKK3) on endothelial barrier permeability, angiogenesis, and tight junctions in LPS-stimulated HPMECs. Methods: RT-qPCR was required for detecting DKK3 and miR-98-3p expression. The angiogenesis of HPMECs was evaluated by tube formation assays. Monolayer permeability of HPMECs was examined by Transwell rhodamine assays. The protein expression of DKK3 and tight junctions in HPMECs was measured via western blotting. Luciferase reporter assay was used to verify the interaction between miR-98-3p and DKK3. Results: LPS treatment inhibited angiogenetic ability while increasing the permeability of HPMECs. DKK3 expression was upregulated while miR-98-3p level was reduced in LPS-treated HPMECs. DKK3 knockdown alleviated HPMEC injury triggered by LPS stimulation. MiR-98-3p targeted DKK3 in HPMECs. Overexpression of miR-98-3p protects HPMECs from the LPS-induced endothelial barrier dysfunction, and the protective effect was reversed by DKK3 overexpression. Conclusions: MiR-98-3p ameliorates LPS-evoked pulmonary microvascular endothelial barrier dysfunction in sepsis-associated ALI by targeting DKK3.