Molecular cloning and characterization of feline cellular genetic sequences homologous to the oncogene of the McDonough strain of feline sarcoma virus

• Published in: Virology (New York, N.Y.) Vol. 141; no. 2; pp. 322 - 327
• Main Authors: Verbeek, Joseph S., de Ruyter, Paul, Bloemers, Henri P.J., Van de Ven, Wim J.M.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.01.1985; Elsevier
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cats - genetics; Cloning, Molecular; DNA Restriction Enzymes; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genes. Genome; Molecular and cellular biology; Molecular genetics; Nucleic Acid Hybridization; Oncogenes; Proto-Oncogenes; Retroviridae - genetics; Sarcoma Viruses, Feline - genetics; Sequence Homology, Nucleic Acid; Fissipedia; Carnivora; Oncovirinae; Nucleotide sequence; Cosmid; Retroviridae; V-Onc gene; Homology; Gene organization; Virus; Vertebrata; Feline sarcoma virus; Mammalia; Oncogenic virus; Type C oncovirus; Animal; DNA; C-Onc gene; Cat; Restriction map; Molecular cloning; Vector
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(85)90266-1

The organization within the cat genome of cellular genetic sequences homologous to the viral oncogene v- fms of the McDonough strain of feline sarcoma virus (SM-FeSV) was determined. Four cosmid clones containing overlapping v- fms homologous cellular DNA inserts representing a contiguous region of cellular DNA of approximately 80 kbp in length have been isolated from a feline cosmid gene library. Within this region of the cat genome, the c- fms genetic sequences are dispersed over a region of around 30 kbp and are interspersed with at least three intervening sequences.