Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers

• Published in: Analytical biochemistry Vol. 164; no. 1; pp. 39 - 43
• Main Authors: Bodhe, A.M., Vartak, H.G., Rele, M.V., Dhamankar, V.S.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.07.1987; Elsevier
• Subjects: Aldose-Ketose Isomerases; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carbohydrate Epimerases - isolation & purification; casein; Dialysis; dialyzable fluorescent markers; Electrophoresis, Polyacrylamide Gel; enzymes; Enzymes and enzyme inhibitors; Fluorescamine; Fluorescence; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; gel electrophoresis; General aspects, investigation methods; peptides; Plant Proteins - isolation & purification; Plants - analysis; polyacrylamide gel electrophoresis; protein purification; proteins; Serum Albumin, Bovine - isolation & purification; Streptomyces - enzymology; Subtilisins - antagonists & inhibitors; protein purification; polyacrylamide gel electrophoresis; fluorescamine; dialyzable fluorescent markers; Proteins; Fluorescent tracer; Purification; Localization; Gel electrophoresis; Enzyme
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(87)90364-2

A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped in locating the proteins. The located desired protein could subsequently be purified by extraction.