In vivo and in vitro pharmacological activity of Aristolochia tagala (syn: Aristolochia acuminata) root extracts

• Published in: Pharmaceutical biology Vol. 49; no. 11; pp. 1210 - 1214
• Main Authors: Battu, Ganga R., Parimi, Ravi, Chandra Shekar, Kottapalli B.
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01.11.2011; Taylor & Francis
• Subjects: Acetates - chemistry; Alkanes - chemistry; Animals; Anti-inflammatory activity; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Aristolochia - chemistry; Calcimycin - pharmacology; Calcium Ionophores - pharmacology; Carrageenan; Cell Line; Dinoprostone - metabolism; Disease Models, Animal; Dose-Response Relationship, Drug; Ethanol - chemistry; Female; Inflammation - chemically induced; Inflammation - prevention & control; Interferon-gamma - pharmacology; kaempferol; Leukotriene B4 - metabolism; LTB; Macrophages - drug effects; Macrophages - metabolism; Male; Mice; PGE; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Roots; Plants, Medicinal; Rats; Rats, Wistar; Time Factors
• ISSN: 1388-0209; 1744-5116
• DOI: 10.3109/13880209.2011.589855

Context: Aristolochia tagala Cham. (syn: Aristolochia acuminata Lam.) (Aristolochiaceae), known as Nallayishwari in Telugu, has been of interest to researchers because of its traditional uses for treating rheumatic pains and fever. Objective: The anti-inflammatory activity of the petroleum ether, ethyl acetate, and ethanol extracts of A. tagala roots were investigated for the first time. Materials and Methods: In vivo and in vitro anti-inflammatory effects were investigated employing the carrageenan-induced hind paw edema in rats and the macrophage cell line RAW264.7 stimulated with proinflammatory stimuli (lipopolysaccharide interferon γ or the calcium ionophore A23187) to determine PGE2 or LTB4 release, respectively. Results: All the extracts exhibited anti-inflammatory effects which were found to be significant (p < 0.001) at 200 and 400 mg/kg, p.o, in rats tested and the ethyl acetate extract inhibited the induction of PGE2 with IC50 = 39.1 mg mL−1 and LTB4 with IC50 = 29.5 mg mL−1. Discussion and conclusion: These findings demonstrate that the A. tagala roots have excellent anti-inflammatory activity and validate the traditional indications of this plant in its origin country.