Modelling of chemical reactions catalysed by membrane-bound enzymes. Determination and significance of the kinetic constants

A model of multiphasic systems, based on the assumption of zero-order partition of substrates and products into the membranes, is applied to reversible mono-substrate and bi-substrate reactioons catalysed by membrane-bound enzymes. Apart from replacement of single-phase kinetic constants by apparent...

Full description

Saved in:
Bibliographic Details
Published inBiochimica et biophysica acta Vol. 995; no. 2; pp. 151 - 159
Main Authors Heirwegh, Karel P.M., Meuwissen, Jules A.T.P., Van den Steen, Peter, De Smedt, Humbert
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 06.04.1989
Elsevier
North-Holland
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:A model of multiphasic systems, based on the assumption of zero-order partition of substrates and products into the membranes, is applied to reversible mono-substrate and bi-substrate reactioons catalysed by membrane-bound enzymes. Apart from replacement of single-phase kinetic constants by apparent kinetic constants, the derived kinetic expressions are formally identical with those for corresponding single-phase systems. The model confers to the apparent kinetic constants an experimentally verifiable meaning. For full characterization of membrane-kinetic systems, experiments at various concentrations of enzyme-embedding phospholipid are required. Extrapolation to zero phospholipid concentration of each K m app then yields the corresponding true kinetic constant characteristic of the membrane-bound enzyme and also provides a technique for determination of the membrane-partition constants. The procedure implies that the phospholipid content should be assayed for full characterization of membrane-bound enzymes. If, for practical reasons, the assays have to be limited to a single enzyme concentration, correction of the apparent kinetic constants is still possible provided the phospholipid concentration and the partition constants of the reactants are known. The model has permitted prediction of a number of previous observations reflecting the multiphasic nature of the systems. The assumptions, underlying the model, and their implications are examined as well as some commonly used experimental designs for determination of the type of enzymic site.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0167-4838
0006-3002
1879-2588
1878-2434
DOI:10.1016/0167-4838(89)90074-5