A reagentless, disposable and multiplexed electronic biosensing platform: Application to molecular logic gates

• Published in: Biosensors & bioelectronics Vol. 26; no. 6; pp. 3077 - 3080
• Main Authors: Xiang, Yun, Qian, Xiaoqing, Zhang, Yuyong, Chen, Ying, Chai, Yaqin, Yuan, Ruo
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.02.2011; Elsevier
• Subjects: Adenosine Triphosphate - analysis; Aptamers; Aptamers, Nucleotide - genetics; Base Sequence; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensing Techniques - statistics & numerical data; Biosensors; Biotechnology; Carbon; Electrochemical Techniques; Electrodes; Fundamental and applied biological sciences. Psychology; Gold; Logic; Logic gates; Metal Nanoparticles; Methods. Procedures. Technologies; Molecular Weight; Multiplexed detection; Point-of-care; Proteins - analysis; Thrombin - analysis; Various methods and equipments; Logic gates; Biosensors; Multiplexed detection; Point-of-care; Aptamers; Disposable; Point-of care; Biosensor; Electronics; Logic gate; Aptamer; Detection; Application
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2010.12.010

The construction of a reagentless, sensitive, disposable and multiplexed electronic sensing platform for one-spot simultaneous determination of biomolecules with significant difference in size (proteins and small molecules) is described. The sensing surface is fabricated by the hybridization of two types of redox-tags conjugated aptamers with the corresponding complementary DNAs, which are self-assembled on a gold nanoparticle-modified screen printed carbon electrode. The presence of the target analytes leads to the release of the tagged signaling aptamers from the sensing surface, and the surface-remained tags exhibit well-resolved peaks, whose positions and sizes reflect the identities and concentrations of the target analytes, respectively. The application of the proposed sensing platform for molecular logic gate operations is also demonstrated.