Use of Cordia dichotoma bark in the treatment of ulcerative colitis

• Published in: Pharmaceutical biology Vol. 49; no. 8; pp. 850 - 855
• Main Authors: Ganjare, Anjali B., Nirmal, Sunil A., Rub, Ruksana A., Patil, Anuja N., Pattan, Shashikant R.
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01.08.2011; Taylor & Francis
• Subjects: Animals; Antioxidants - metabolism; Antioxidants - pharmacology; Antioxidants - therapeutic use; Benzothiazoles - metabolism; Biphenyl Compounds - metabolism; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - pathology; Colon - pathology; Cordia; Cordia dichotoma; Free Radical Scavengers - metabolism; Free Radical Scavengers - pharmacology; histopathology; Male; malondialdehyde; Malondialdehyde - blood; Malondialdehyde - metabolism; Medicine, Ayurvedic; Mice; myeloperoxidase; Peroxidase - blood; Peroxidase - metabolism; Phenols - analysis; Phytotherapy; Picrates - metabolism; Plant Bark - chemistry; Plant Extracts - metabolism; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Sulfonic Acids - metabolism; ulcerative colitis
• ISSN: 1388-0209; 1744-5116
• DOI: 10.3109/13880209.2010.551539

Context: The plant Cordia dichotoma Forst. f. (Boraginaceae) is commonly known as "Bhokar" in Marathi. This tree species has been of interest to researchers because traditionally its bark is reported in the treatment of ulcer and colic pain. Objective: The present work was undertaken to validate its folk use in the treatment of ulcerative colitis (UC) by using scientific methods. Materials and methods: Dried bark powder was extracted with methanol and this crude methanol extract was fractionated using various solvents. These fractions were tested for effectiveness against UC. Macroscopical study and histopathology of the colon, level of myeloperoxidase (MPO) and malondialdehyde (MDA) in colon and blood were studied for the assessment of the activity. Antioxidant activity of these fractions was screened by using various methods. Results: Animals treated with the methanol fraction of the crude methanol extract showed lower pathological scores and good healing. This fraction reduced MPO and MDA levels significantly in blood and tissue. It showed antioxidant potential [in DPPH (1,1-diphenyl-2-picrylhydrazyl) assay IC50 value is 26.25; trolox equivalent (TE) antioxidant capacity µg/ml TE/g of plant material on dry basis in ABTS (2,2′-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid) and FRAP (ferric reducing antioxidant potential) assay is 2.03 and 2.45, respectively]. The fraction contains a high level of phenolics. Discussion and conclusion: The methanol fraction of crude methanol extract of C. dichotoma bark is effective in the treatment of UC.