HSV-1-inducible proteins bind to NF-κB-like Sites in the HSV-1 genome

• Published in: Virology (New York, N.Y.) Vol. 189; no. 2; pp. 750 - 756
• Main Authors: Rong, Bing L., Libermann, Towia A., Kogawa, Keiko, Ghosh, Sankar, Cao, Li-Xian, Pavan-Langston, Deborah, Dunkel, Edmund C.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.08.1992; Elsevier
• Subjects: Base Sequence; Biological and medical sciences; Consensus Sequence; DNA, Viral - genetics; DNA-Binding Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Viral; Genetics; herpes simplex virus 1; Humans; In Vitro Techniques; Microbiology; Molecular Sequence Data; NF-kappa B - metabolism; Nuclear Proteins - metabolism; Oligodeoxyribonucleotides - chemistry; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid; Simplexvirus - genetics; Viral Proteins - genetics; Virology; Virus; Proteins; Herpesvirus hominis 1; Herpesviridae; Alphaherpesvirinae; Molecular interaction; Binding site
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(92)90599-K

Several putative NF-κB-binding sites in the ICPO and Vmw65 herpes simplex virus type-1 (HSV-1) genes have been identified. Oligonucleotides encoding some of these sites bind specifically to purified NF-κB protein and an NF-κB-like protein in nuclear extracts of phorbol ester- or cycloheximide-induced human embryonic lung (HEL) cells. HSV-1 infection of HEL cells induced a nuclear factor that binds specifically to κB sites in the ICPO and Vmw65 gene regions and comigrates with complexes formed by purified NF-κB. The HSV-1-inducible nuclear factor bound to the authentic immunoglobulin (Ig) κB site. Transient expression of chloramphenicol acetyltransferase (CAT) plasmids containing two copies of the Ig κB site upstream of the c-fos promoter (KB2CAT) showed little activity in HEL cells. HSV-1 infection of κB 2-CAT-transfected HEL cells, however, induced a dramatic increase in CAT activity; mutation in the NF-κB-binding site of κB 2CAT abolished the inducibility of CAT gene expression. Our results demonstrate that the HSV-1 ICPO and Vmw65 gene regions contain binding sites for NF-κB, and that HSV-1-inducible proteins bind to NF-κB-like sites in the HSV-1 genome.