The effects of temperature on the cytochrome P-450 system of thermally acclimated bluegill

• Published in: Comparative biochemistry and physiology. C, Comparative pharmacology Vol. 80; no. 1; pp. 135 - 139
• Main Authors: Karr, Susan W., Reinert, Robert E., Wade, Adelbert E.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 1985; New York, NY Pergamon Press
• Subjects: Acclimatization; Animals; Aryl Hydrocarbon Hydroxylases - metabolism; Benzopyrene Hydroxylase - metabolism; Biological and medical sciences; Body Weight; Cytochrome P-450 Enzyme System - metabolism; Electrophoresis, Polyacrylamide Gel - methods; Enzymes. Coenzymes. Vitamins. Pigments; Fishes - metabolism; Freshwater; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Kinetics; Lepomis macrochirus; Liver - enzymology; Liver - physiology; Metabolisms and neurohumoral controls; Microsomes, Liver - enzymology; Organ Size; Temperature; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Lepomis macrochirus; Freshwater environment; Vertebrata; Temperature; Enzyme; Cytochrome P450; Pisces; Metabolism; Adaptation
• ISSN: 0306-4492; 0742-8413
• DOI: 10.1016/0742-8413(85)90144-6

1. The effects of temperature acclimation at 10, 20 and 30°C on the concentration and activity of the mixed function oxidase system in bluegill are as follows. 2. Liver weight/body weight varied inversely with temperature. 3. Significant ( P < 0.05) differences in concentration of cytochrome P-450 of hepatic microsomes were seen and varied inversely with temperature. 4. Benzo( a)pyrene hydroxylase activity tested in vitro at incubation temperatures of 10, 20 and 30°C showed significant differences in K m, but no differences in V max. 5. SDS polyacrylamide gel electrophoresis revealed some quantitative differences in cytochrome P-450 isozymes between groups.