Inhibition of moloney murine leukemia virus reverse transcriptase by α-anomeric oligonucleotides

• Published in: Biochemical and biophysical research communications Vol. 161; no. 3; pp. 1184 - 1190
• Main Authors: Lavignon, Marc, Bertrand, Jean-Rémi, Rayner, Bernard, Imbach, Jean-Louis, Malvy, Claude, Paoletti, Claude
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 30.06.1989; Elsevier
• Subjects: Base Sequence; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genetics; Isomerism; Kinetics; Microbiology; Moloney murine leukemia virus - enzymology; Oligodeoxyribonucleotides - pharmacology; Reverse Transcriptase Inhibitors; Structure-Activity Relationship; Templates, Genetic; Virology; BSA; RT; TCA; HIV; M-MLV; DTT; AIDS; oligo; Performance evaluation; Molecular hybridization; Oncovirinae; Radiolabelling; Gel electrophoresis; Enzyme; Retroviridae; Dose activity relation; Virus; Enzymatic activity; Type C oncovirus; DNA polymerase RNA-dependent; Nucleotide; Murine leukemia virus; Oligonucleotide; Kinetics; Inhibition
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(89)91367-3

After parallel hybridization to complementary template RNA, α-anomeric oligonucleotides are not primers for Moloney murine leukemia virus reverse transcriptase. As can be expected they are competitors of classical primer oligonucleotides (β-anomeric). They therefore inhibit the RNA dependent DNA polymerase activity of Moloney murine leukemia virus reverse transcriptase with either homopolymeric or heteropolymeric substrates. Non complementary α-oligonucleotides display no inhibitory activity. α-Oligonucleotides are therefore potentiel candidates for inhibition of retroviral reverse transcriptases by interference with the primer binding sites.